白来航蛋鸡产蛋前后肝转录组功能分析

doi:10.11843/j.issn.0366-6964.2017.09.007

Abstract

Abstract:

The objective of this study was to analyze the changes of the liver metabolic function and its effects on nutrients conversion and utility based on differentially expressed gene analysis in the liver. Full-sib White Leghorn layer hens for one week before and after first egg laying were chose for liver sampling. For analyzing and screening the important genes involved in the nutrient metabolic pathway in the liver, the transcriptome data was obtained by RNA-seq technology and bioinformatics analysis, and the gene expression differences was compared before and after the first egg laying. Two hundred and twenty-two differentially expressed genes with more than 2-fold changed were obtained by the transcriptomics sequencing (P<0.05). GO enrichment analysis and KEGG pathway analysis of differentially expressed genes showed that differentially expressed genes obtained in this study mainly involved in lipid metabolism and tryptophan metabolism.In this study, there were some function-unknown differentially expressed genes were found, such as Ring finger protein 186 (RNF186). We obtained the differentially expressed genes in liver from White Leghorn hens before and after first eggs laying and analyzed the changes of metabolism pathway of nutrients in the liver. These results proved that the liver metabolism pathway of nutrients were changed by these differentially expressed genes and the changes might improve the nutrients utilization of the feed after first egg laying.

CLC Number:

S831.2

LI Hui-feng, ZHANG Zhen, ZHU Wen-jin, ZHANG Tong-yu, GUO Wen-jie, CAI Yong-qiang, ZHU Zhi-wei, ZHANG Li-huan. Functional Analysis of Liver Transcriptome before and after White Leghorn Hen Begin Laying Eggs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(9): 1624-1634.

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Functional Analysis of Liver Transcriptome before and after White Leghorn Hen Begin Laying Eggs

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